ecotropic packaging helper plasmid pcleco Search Results


fugene hd transfection reagent  (Promega)
90
Promega fugene hd transfection reagent
Supernatants and cell lysates from 293FT cells were analysed 64h after <t>transfection</t> with either empty vector (pMiGR1), murine neutrophil elastase (mNE), human neutrophil elastase (hNE) or mNE/hNE mutants G185R (mNEG185R/hNEG185R) or S97L (mNES97L/ hNES97L). (A) expression of murine neutrophil elastase (mNE) and the ER-stress markers BIP (GRP78/HASP5) and PDI in cell lysates and supernatants, (B) expression of human neutrophil elastase (hNE) in cell lysates and supernatant (the same samples as in (A) were rerun on a separate gel and probed with a human-specific anti-hNE antibody). Samples corresponding to 20μg cell lysate or 20μl of supernatant were separated by SDS-PAGE, transferred onto nitrocellulose membranes and probed for the antibodies indicated. GAPDH served as loading control. Data represent one of two independent experiments.
Fugene Hd Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecotropic+packaging+helper+plasmid+pcleco/fugene+hd/pmc05152902-173-26-30
Average 90 stars, based on 1 article reviews
fugene hd transfection reagent - by Bioz Stars, 2026-09
90/100 stars
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293t phoenix ecotropic packaging cell line  (ATCC)
99
ATCC 293t phoenix ecotropic packaging cell line
Supernatants and cell lysates from 293FT cells were analysed 64h after <t>transfection</t> with either empty vector (pMiGR1), murine neutrophil elastase (mNE), human neutrophil elastase (hNE) or mNE/hNE mutants G185R (mNEG185R/hNEG185R) or S97L (mNES97L/ hNES97L). (A) expression of murine neutrophil elastase (mNE) and the ER-stress markers BIP (GRP78/HASP5) and PDI in cell lysates and supernatants, (B) expression of human neutrophil elastase (hNE) in cell lysates and supernatant (the same samples as in (A) were rerun on a separate gel and probed with a human-specific anti-hNE antibody). Samples corresponding to 20μg cell lysate or 20μl of supernatant were separated by SDS-PAGE, transferred onto nitrocellulose membranes and probed for the antibodies indicated. GAPDH served as loading control. Data represent one of two independent experiments.
293t Phoenix Ecotropic Packaging Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecotropic+packaging+helper+plasmid+pcleco/293T/pmc04362235-353-18-24
Average 99 stars, based on 1 article reviews
293t phoenix ecotropic packaging cell line - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

Image Search Results


Supernatants and cell lysates from 293FT cells were analysed 64h after transfection with either empty vector (pMiGR1), murine neutrophil elastase (mNE), human neutrophil elastase (hNE) or mNE/hNE mutants G185R (mNEG185R/hNEG185R) or S97L (mNES97L/ hNES97L). (A) expression of murine neutrophil elastase (mNE) and the ER-stress markers BIP (GRP78/HASP5) and PDI in cell lysates and supernatants, (B) expression of human neutrophil elastase (hNE) in cell lysates and supernatant (the same samples as in (A) were rerun on a separate gel and probed with a human-specific anti-hNE antibody). Samples corresponding to 20μg cell lysate or 20μl of supernatant were separated by SDS-PAGE, transferred onto nitrocellulose membranes and probed for the antibodies indicated. GAPDH served as loading control. Data represent one of two independent experiments.

Journal: PLoS ONE

Article Title: Characterisation of Neutropenia-Associated Neutrophil Elastase Mutations in a Murine Differentiation Model In Vitro and In Vivo

doi: 10.1371/journal.pone.0168055

Figure Lengend Snippet: Supernatants and cell lysates from 293FT cells were analysed 64h after transfection with either empty vector (pMiGR1), murine neutrophil elastase (mNE), human neutrophil elastase (hNE) or mNE/hNE mutants G185R (mNEG185R/hNEG185R) or S97L (mNES97L/ hNES97L). (A) expression of murine neutrophil elastase (mNE) and the ER-stress markers BIP (GRP78/HASP5) and PDI in cell lysates and supernatants, (B) expression of human neutrophil elastase (hNE) in cell lysates and supernatant (the same samples as in (A) were rerun on a separate gel and probed with a human-specific anti-hNE antibody). Samples corresponding to 20μg cell lysate or 20μl of supernatant were separated by SDS-PAGE, transferred onto nitrocellulose membranes and probed for the antibodies indicated. GAPDH served as loading control. Data represent one of two independent experiments.

Article Snippet: Retroviral particles were produced by transient transfection of the retroviral expression constructs together with the packaging plasmid pCLEco into the ecotrophic packaging cell line Phoenix using Fugene HD transfection reagent (Promega, Madison, WI, USA).

Techniques: Transfection, Plasmid Preparation, Expressing, SDS Page, Control